Linkage Biosciences

SAN FRANCISCO, California, November 18, 2009 – Linkage Biosciences, Inc., a privately held molecular diagnostics company, recently unveiled LinkSēq™ -- a new one-step method for Human Leukocyte Antigen (HLA) testing -- at the annual meeting of the American Society of Histocompatibility and Immunogenetics (ASHI) in San Francisco. Much faster than other HLA testing methods currently available, LinkSēq™ is a proprietary sequence-specific priming (SSP) chemistry that can be analyzed utilizing real-time PCR platforms.

"Lab professionals have been waiting for a faster method of HLA testing for years and we’re excited to be able to offer a solution that not only saves precious time and labor, but also uses equipment that many labs already own, eliminating upfront costs," said Zachary Antovich, President and CEO of Linkage Biosciences. "We are very pleased by the positive reaction from leading medical labs to the LinkSēq™ product line and look forward to helping them achieve their goals. We are addressing a significant market opportunity for HLA testing, estimated to be in excess of $270 million globally."

HLA genes encode cell-surface proteins that are essential elements of the immune system. These proteins are a major factor influencing immunity, autoimmunity, genetic disease, and tissue transplantation. Current methods of testing typically rely on gel electrophoresis or nucleic acid probing techniques. These methods require substantial time, labor, and skilled laboratory workers; even then, data analysis is often complicated and time consuming.

With LinkSēq™, the actual labor time is under 15 minutes with results ready in about 90 minutes, a significant improvement compared to the 4-6 hours typically required by other HLA testing methods. In addition to saving time, LinkSēq’s single step process reduces the potential for contamination since samples are handled only once and then sealed in reaction plates.

"LinkSēq™ is a major improvement in the workflow for HLA typing. I like the quick turnaround times and easy set-up; just add DNA to a tube and pipette into the reaction plate. Very straightforward," said Dr. Elizabeth Trachtenberg, Director, HLA/Immunogenetics Laboratory, Children’s Hospital and Research Center Oakland.

LinkSēq’s innovative chemistry, an end-point PCR process, utilizes SYBR® Green to detect HLA genes. Once the PCR amplification process is completed, SYBR® Green fluorescence is detected via the real-time PCR platform to identify specific HLA genotypes. Gels and probing steps are eliminated and data analysis is simplified with the company’s SureTyper™ software, automating the entire process.

"LinkSēq™ chemistry combined with SYBR® analysis provides clear and defined results. Scale-up is easy, too, since the trays can be run on common thermal cyclers prior to analysis. This is an attractive feature," said Dr. Shalini Pereira, Director, Clinical Immunogenetics Laboratory, Seattle Cancer Care Alliance.

SAN FRANCISCO, California FEBRUARY 4, 2009 Linkage Biosciences Inc., a developer of molecular diagnostic products designed to streamline complex genetic testing, has completed a Series A financing led by Greenhouse Capital Partners and James R. Fisher, managing member of Fisher Capital Corp. II LLC, a firm that works exclusively with private equity firm Kohlberg Kravis Roberts & Co..

The $2 million Series A funding round will be used to continue the development and marketing of Linkage Biosciences’ line of diagnostic products. The company’s proprietary molecular diagnostics technologies and innovations will address human leukocyte antigen (HLA) typing for organ transplantation, while further development is underway on a portfolio of products in other areas of transplantation and various complex genetic disorders.

“Our unique advantages are speed and simplicity while achieving superior accuracy,” said Zachary Antovich, president and CEO of Linkage Biosciences. “Our tests generate results much faster, with far less effort, than anything else available in the marketplace. We are pleased to have the support and experience of Greenhouse Capital and Jim Fisher as we begin to commercialize our products and expand our presence in the marketplace.”

“Linkage Biosciences’ initial testing has yielded exceptional results in tissue typing applications, and we now see the company moving to a second level of growth and development, proving that it can create an entire portfolio of molecular diagnostic products to bring to market,” said Peter Henig, managing partner of Greenhouse Capital. “We are thrilled to be an investment partner in the company’s growth and execution at this time. We believe this is an ideal time for Linkage Biosciences to initiate its market rollout strategy as the technology has surpassed significant milestones.”

The company also announced that Rebecca Bishop and Pascal Villiger have joined the Board of Directors. Ms. Bishop is currently a manager with Affymetrix, Inc. and Mr. Villiger is an investment professional within the private equity division of CalSTRS.

CHICO, California MAY 13, 2008 Improve lives, earn profits, create jobs. With these goals in mind, three companies made the grade as the “Most Valuable Presenters” at the 2008 Venture Vineyard Early Stage Conference and Santa Rosa Innovation Investment Forum at the Hilton Sonoma Wine Country Hotel in Santa Rosa, California on April 30, 2008.

KeyTech LLC (www.KeyTechLLC.com) of San Rafael was selected as the “North Bay Region Most Valuable Presenter,” and Evolve Game Studios (www.evolvegamestudios.com) of Rohnert Park, was selected as “Most Valuable Presenter” among “Regional Companies to Watch.,”

AspenAir (www.aspenairinside.com) of Martinez and Linkage Biosciences, (www.linkagebio.com), of San Francisco, shared the overall award of “Most Valuable Presenters.”

-AspenAir is tackling the indoor air quality market with a line of patented electronic air filtration products that fit into existing HVAC systems.

-Evolve Game Studios is creating massively, multiplayer online games for the casual gamer.

-KeyTech captured the attention of the audience and panelists with a patented, kiosk-based solution to a problem that many have: 24/7 key duplication.

-Linkage Biosciences, part of the fast growing molecular diagnostics segment, is applying their proprietary technology to the development of a new method of genetic analysis for organ transplantation.

More than 30 companies competed for the honor during a day packed with presentations from early stage companies from throughout the western U.S seeking qualified equity capital.

Each company participated in rigorous screening and coaching prior to showcasing the merits of their ventures during a 15 minute presentation session before panels of active, accredited angel and venture capital investors and industry experts. The top six were selected to compete in the finalist showcase.

“The companies selected, ranging from making keys to purifying air to genetics, demonstrate the scope of the problems being solved by companies that present at our conferences,” said Jon Gregory, President and CEO of Golden Capital Network. “Between the local companies showcased and those that came from the rest of the western United States, it’s clear that the slowing economy hasn’t slowed down innovation.”

The Most Valuable Presenters were selected by a panel of investors including:

-Steve Rothenberg, Band of Angels;

-Cliff Detz, North Bay Angels;

-Mike Rizzo, Five Star Bank; and

-Peter Henig, Greenhouse Capital Partners.

The other presenting companies selected as finalists include:

-DualPoint Wireless (www.dualpoint.com) - GPS Tracking, Navigation, with RFID integration;

-Tax Lifeboat (www.taxlifeboat.com) - automated tax defense; and

-Rexanto (email: info@rexanto.com) - reducing the cost of meds for seniors.

For more information about the conference, a complete list of presenting companies and participating investors, please visit: http://www.goldencapital.net/Events/

ABOUT GOLDEN CAPITAL NETWORK

Golden Capital Network is a non-profit networking, training and consulting group that fosters growth entrepreneurship and early stage investing as an engine for economic growth.

Since 1999, GCN has coached and showcased more than 1000 companies to over 500 active, accredited angel and VC investors at our events. These are the largest and most robust events of their type. The GCN event formula has resulted in over $1 billion raised by presenting companies. More information on Golden Capital is available at www.goldencapital.net.

Linkage Biosciences Inc., a developer of molecular diagnostics products designed to streamline complex genetic testing, has completed a financing round led by Greenhouse Capital Partners LP, a seed-stage venture capital firm, which brings the total funding raised thus far to approximately $1 million. Linkage Biosciences said the new funds will be used to accelerate development of its initial line of diagnostic products for solid organ and stem cell transplantation.

“Our strategy is to deliver products that solve important clinical needs in ways that are faster and less labor intensive than ever before,” said Zachary Antovich, President and CEO of Linkage Biosciences. “With our proprietary technology and strong business and scientific teams, we are confident we have the resources to make a lasting impact in our market.”

Linkage Biosciences’ Scientific Advisory Board includes:

• F. Carl Grumet, M.D., Emeritus Professor of Pathology and Emeritus Director Stanford Histocompatibility Laboratory, Stanford University
• Clifford Lowell, M.D., Ph.D., Professor and Chair of the Department of Laboratory Medicine, University of California, San Francisco
• Rade Drmanac, Ph.D., Chief Science Officer of Complete Genomics
• David Ginzinger, Ph.D., President DGG Consulting

“A pioneering entrant in the diagnostics-testing space, Linkage Biosciences has what we believe is a rare and promising opportunity to re-define today’s standards and significantly grow this market,” said Peter D. Henig, Managing Partner of Greenhouse Capital Partners. “We look forward to helping the company enhance its technology development and time to market, and to also structurally prepare them for subsequent financing rounds.”

Linkage Biosciences also announced that Mr. Henig has joined the company’s board of directors.